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  1. The high viscosity of Matrigel leads to high capillary number, complicating droplet generation. This can be resolved by tuning flow rate, interfacial tension, or device geometry. 
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  2. Laser Induced Deep Etching (LIDE®), developed by LPKF, is a maskless laser processing method capable of patterning glass microstructures similar to microfluidics created by PDMS soft lithography. Here, we demonstrate a self-digitized droplet microfluidics chip with high aspect-ratio features and fine resolution via the LIDE® technology. LIDE® provides the means to translate microfluidic designs into glass in a process suitable for low-cost and high-volume manufacturing. 
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